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- Title
Differential regulation of insulin-like growth factor binding protein-1 and -2 by insulin in the baboon (Papio anubis) endometrium.
- Authors
Fleming, Steven D.; Fazleabas, Asgerally T.; Bell, Stephen C.
- Abstract
Background: The purpose of this study was to examine the effect of insulin on expression and synthesis of IGFBP-1 and IGFBP-2 in the baboon endometrium in vitro. Methods: Baboon endometrial explants collected from cycling, ovariectomized, steroid-treated, simulated-pregnant and pregnant animals were cultured for 48 h in the presence or absence of insulin, with or without estradiol, progesterone and hCG. Results: Insulin clearly inhibited IGFBP-1 production and mRNA expression in a time- and dose-dependent manner, whereas IGFBP-2 synthesis was not significantly affected. The inhibitory effects of insulin on IGFBP-1 were more evident in explants of non-pregnant tissue or tissue away from the implantation site. In the absence of insulin, synthesis of IGFBP-1 was induced in explants with low levels of de novo synthesis whereas IGFBP-2 synthesis was inhibited. This effect was potentiated by steroids and hCG in the explant cultures. Conclusion: Insulin differentially regulates endometrial IGFBP-1 and IGFBP-2 secretion in the baboon.
- Subjects
INSULIN; INSULIN-like growth factor-binding proteins; ENDOMETRIUM; TISSUE culture; BABOON anatomy
- Publication
Reproductive Biology & Endocrinology, 2008, Vol 6, p1
- ISSN
1477-7827
- Publication type
Article
- DOI
10.1186/1477-7827-6-6