We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers.
- Authors
Lázaro-Castellanos, Jesús Omar; Mata-Rosas, Martín; González, Dolores; Arias, Salvador; Reverchon, Frédérique
- Abstract
In vitro propagation protocols were established for endangered species of cacti Mammillaria hernandezii, M. dixanthocentron, and M. lanata. In vitro-germinated seedlings were used as the explant source. Three explant types were evaluated as apical, basal, and lateral stem sections. Shoot multiplication was achieved using Murashige and Skoog (MS) medium supplemented with benzyladenine, kinetin, meta-topolin, and thidiazuron in equimolar concentrations (0.0, 0.4, 1.1, 2.2, 4.4, and 8.9 μM). Shoot regeneration was obtained primarily in the lateral stem section explants. In M. hernandezii, an average of 7.4 shoots was regenerated in MS medium with 2.2 μM meta-topolin. M. dixanthocentron and M. lanata averaged 16.7 and 17.9 shoots/explant, respectively, in MS medium supplemented with 1.1 μM meta-topolin. Rooting occurred in MS medium without growth regulators. Three in vitro culture cycles were performed to validate the propagation protocols and to verify genetic stability. Shoots were collected in each cycle and genomic DNA was extracted. Amplified microsatellites were used to compare each genotype with its respective donor plant. Polymorphic information content analysis showed low levels of intra-clonal polymorphisms—M. hernandezii 0.04 and M. dixanthocentron and M. lanata both 0.12. More than 95% of the plants were successfully acclimatized in the greenhouse. After 12 months, plants of M. hernandezii reached the flowering stage; M. dixanthocentron and M. lanata flowered at 24 mo.
- Subjects
MAMMILLARIA (Cactus); PLANT micropropagation; PLANT species; PLANT breeding; PLANT morphology; ROOTING of plant cuttings
- Publication
In Vitro Cellular & Developmental Biology Plant, 2018, Vol 54, Issue 5, p518
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-018-9908-z