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- Title
Genome-wide analysis of key salinity-tolerance transporter ( HKT1; 5) in wheat and wild wheat relatives (A and D genomes).
- Authors
Zamani Babgohari, Mahbobeh; Niazi, Ali; Moghadam, Ali; Deihimi, Tahereh; Ebrahimie, Esmaeil
- Abstract
Exclusion of sodium ions from cells is one of the key salinity tolerance mechanisms in plants. The high-affinity cation transporter ( HKT1; 5) is located in the plasma membrane of the xylem, excluding Na from the parenchyma cells to reduce Na concentration. The regulatory mechanism and exact functions of HKT genes from different genotypic backgrounds are relatively obscure. In this study, the expression patterns of HKT1; 5 in A and D genomes of wheat were investigated in root and leaf tissues of wild and domesticated genotypes using real-time PCR. In parallel, the K/Na ratio was measured in salt-tolerant and salt-sensitive cultivars. Promoter analysis were applied to shed light on underlying regulatory mechanism of the HKT1; 5 expression. Gene isolation and qPCR confirmed the expression of HKT1; 5 in the A and D genomes of wheat ancestors ( Triticum boeoticum, AbAb and Aegilops crassa, MMDD, respectively). Interestingly, earlier expression of HKT1; 5 was detected in leaves compared with roots in response to salt stress. In addition, the salt-tolerant genotypes expressed HKT1; 5 before salt-sensitive genotypes. Our results suggest that HKT1; 5 expression follows a tissue- and genotype-specific pattern. The highest level of HKT1; 5 expression was observed in the leaves of Aegilops, 6 h after being subjected to high salt stress (200 mM). Overall, the D genome allele ( HKT1; 5-D) showed higher expression than the A genome ( HKT1; 5-A) allele when subjected to a high NaCl level. We suggest that the D genome is more effective regarding Na exclusion. Furthermore, in silico promoter analysis showed that TaHKT1; 5 genes harbor jasmonic acid response elements.
- Subjects
SALINITY; CARRIER proteins; WHEAT; WILD wheats; SODIUM ions; GENE expression in plants; POLYMERASE chain reaction; PLANT gene isolation
- Publication
In Vitro Cellular & Developmental Biology Plant, 2013, Vol 49, Issue 2, p97
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-012-9478-4