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- Title
OUL HIPERIMUN: PREPARARE, CARACTERIZARE ȘI ACTIVITATE IMUNOTERAPEUTICĂ ALTERNATIVĂ.
- Authors
Supeanu, Teodora; Tablică, Mădălina; Urducea, Cristina; Dimulescu, Ioana Alina; Sima, Lucica; Chiurciu, Viorica; Oporanu, Mariana; Chiurciu, C.
- Abstract
The research carried out describes one preparation procedure of the monovalent and polyvalent hyper immune eggs, obtained from hens immunized with a bacterial and fungal antigen complex, pathogenic for humans, resistant or sensitive to antibiotics. It was prepared a monovalent antigen of Staphylococcus aureus MRSA strain, isolated from a patient with urinary infection. Lots of laying hens have been formed, at the beginning of the laying period, which were immunized on days, 0, 14 and 28. From the hyper immune eggs obtained the specific immunoglobulin Y (IgY) was extracted and purified by polyethylene glycol precipitation (PEG 6000). Purity analysis was performed by polyacrylamide gel electrophoresis in a denatured system (SDS-PAGE) obtaining a single fraction with highlighting of heavy chains (molecular weight=75KDa) and light chains (molecular weight=25KDa). By the agar gel immune-diffusion assay (IDGA), the identity between analyzed IgY and standard IgY (MyBiosurse) was highlighted. The specificity of IgY antibodies obtained from hyper immune egg yolk was proved by the ELISA immune enzymatic assay, obtaining high titers for each antigen present in the inoculum. The hyper immune eggs were used for preparation of biologically active products from the range IMUNOINSTANT, recommended in the therapy of diseases caused by multi resistant germs.
- Publication
Veterinary Drug / Medicamentul Veterinar, 2019, Vol 13, Issue 1, p84
- ISSN
1843-9527
- Publication type
Article