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- Title
Bisphenol A exposure modifies methylation of imprinted genes in mouse oocytes via the estrogen receptor signaling pathway.
- Authors
Chao, Hu-He; Zhang, Xi-Feng; Chen, Bo; Pan, Bo; Zhang, Lian-Jun; Li, Lan; Sun, Xiao-Feng; Shi, Qing-Hua; Shen, Wei
- Abstract
Bisphenol A (BPA), a synthetic additive used to harden polycarbonate plastics and epoxy resin, is ubiquitous in our everyday environment. Many studies have indicated detrimental effects of BPA on the mammalian reproductive abilities. This study is aimed to test the potential effects of BPA on methylation of imprinted genes during oocyte growth and meiotic maturation in CD-1 mice. Our results demonstrated that BPA exposure resulted in hypomethylation of imprinted gene Igf2r and Peg3 during oocyte growth, and enhanced estrogen receptor (ER) expression at the levels of mRNA and protein. The relationship between ER expression and imprinted gene hypomethylation was substantiated using an ER inhibitor, ICI182780. In addition, BPA promoted the primordial to primary follicle transition, thereby speeding up the depletion of the primordial follicle pool, and suppressed the meiotic maturation of oocytes because of abnormal spindle assembling in meiosis I. In conclusion, neonatal exposure to BPA inhibits methylation of imprinted genes during oogenesis via the ER signaling pathway in CD-1 mice.
- Subjects
BISPHENOL A; METHYLATION; ESTROGEN receptors; CELLULAR signal transduction; POLYCARBONATE resins; GENE expression; LABORATORY mice
- Publication
Histochemistry & Cell Biology, 2012, Vol 137, Issue 2, p249
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-011-0894-z