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- Title
Mechanism of Sanshi decoction inhibits macrophage pyroptosis by inhibiting BRD4/NF-κB/NLRP3 pathway in the treatment of gouty arthritis.
- Authors
PIAO Yong-zhu; QI Ming-ming; NIE Shuang-lian; PAN Guo-xiong; ZHANG Hao; WANG Xin-bo
- Abstract
Objective: To observe the effect of Sanshi decoction on BRD4/NF-κB/NLRP3 pathwaymediated macrophage pyroptosis, so as to elucidate the molecular mechanism of Sanshi decoction in the treatment of gouty arthritis. Methods: THP-1 was induced into macrophages with foboside and the divided into the control group, model group, low-dose, medium-dose, high-dose group of Sanshi decoction, and BRD4 inhibitor group. Except for the control group, the remaining groups were induced with monosodium urate crystals to construct a gouty arthritis cell model. The activity of macrophages was detected by CCK8, the level of macrophage pyroptosis was detected by flow cytometry, the activity of LDH, the content of IL-1β and IL-18 were detected by enzyme-linked immunosorbent assay, and the expression of related proteins in the BRD4/NF-κB/NLRP3 pathway was detected by Western blot. Results: Compared with the control group, macrophage activity was decreased in the model group, and the level of pyroptosis, LDH activity, contents of IL-1β and IL-18, expression levels of BRD4, p-NF-kB p65, NLRP3, Caspase-1 p20, and IL-1β protein were significantly up-regulated, the differences were statistically significant (P<0.05 and P <0.01). Compared with the model group, macrophage activity was up-regulated in the Sanshi Decoction, and the level of pyroptosis, LDH activity, IL-1β and IL-18 contents, expression levels of BRD4, p-NF-kB p65, NLRP3, Caspase-1 p20, and IL-1β protein were significantly decreased with statistically significant differences (P<0.05 and P <0.01). Conclusion: Sanshi decoction inhibits macrophage pyroptosis by inhibiting BRD4/NF-κB/NLRP3 pathway activation, thus improving the inflammation level of gouty arthritis.
- Subjects
URATES; PYROPTOSIS; MACROPHAGES; ENZYME-linked immunosorbent assay; ARTHRITIS
- Publication
Journal of Hainan Medical University, 2023, Vol 29, Issue 24, p18
- ISSN
1007-1237
- Publication type
Article