We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
固定化青霉素酰化酶对荧光假单胞菌群体感应的抑制作用.
- Authors
申 悦; 劳敏军; 王当丰; 崔方超二; 林 洪為; 李婷婷匚; 励建荣
- Abstract
Quorum sensing in spoilage bacteria of aquatic products is one of the reasons to accelerate the spoilage of aquatic products. In recent years, due to the use of chemical preservatives, many spoilage bacteria have developed resistance, and their safety also worries consumers} so the research on safe and efficient biological preservatives is an important topic in aquatic preservation. In this study, we tried to use immobilized penicillin acylase to inhibit the quorum sensing of Pseudomonas fluorescens. The quenching effect of immobilized penicillin acylase on Pseudomonas fluorescens AHLs was verified by CV026; the effect of enzyme on biofilm was studied by optical microscope and scanning electron microscope; the activity of extracellular protease was verified by milk agar plate; the interaction between enzyme and different AHLs was predicted by molecular docking. The results showed that the immobilized penicillin acylase could reduce the content of AHLs in Pseudomonas fluorescens, thus reducing the production of purpurin in Pseudomonas fluo-rescens CV026 and the formation of Pseudomonas fluorescens biofilm and extracellular polymer. At the same time, it also interfered with the expression of extracellular protease. When the enzyme mass concentration was 15 mg/mL} no obvious protein inhibition circle could be observed. Molecular docking results showed that the enzjrme could successfully dock with both short chain and long chain signaling molecules, but preferred to long chain AHLs. Compared with Cz-HSL: Cg-HSL was completely wrapped by amino acid residues around the binding capsule and connected with each other by hydrogen bonds, with more hydrogen bonds and stronger binding. It can be concluded that the immobilized penicillin acylase has good quorum sensing quenching activity and can be used as a new preservative for aquatic products.
- Subjects
ACYL-homoserine lactones; PSEUDOMONAS fluorescens; OPTICAL microscopes; QUORUM sensing; AMINO acid residues; SCANNING electron microscopes; MILK proteins; AGAR
- Publication
Journal of Chinese Institute of Food Science & Technology, 2022, Vol 22, Issue 10, p77
- ISSN
1009-7848
- Publication type
Article
- DOI
10.16429/j.1009-7848.2022.10.009