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- Title
ACE2‐enriched extracellular vesicles enhance infectivity of live SARS‐CoV‐2 virus.
- Authors
Tey, Sze Keong; Lam, Hoiyan; Wong, Samuel Wan Ki; Zhao, Hanjun; To, Kelvin Kai‐Wang; Yam, Judy Wai Ping
- Abstract
Extracellular vesicles (EVs) are nanoscale vesicles naturally released from cells that are delimited by a lipid bilayer and cannot replicate (Van Niel et al., 2018). ACE2-enriched extracellular vesicles enhance infectivity of live SARS-CoV-2 virus For the multicycle study, SARS-CoV-2 (1 × 10 SP 3 sp PFU/ml) was premixed in ratio 1:1 with or without EVs (EVs original concentration: 1 × 10 SP 11 sp particles/ml, diluted to 1:2, 1:4, 1:8 and 1:32) (100 l of virus + 100 l of diluted EVs) for 1 h in room temperature, and was then added to Vero E6 cells for incubation at 37°C. Our data revealed a distinct role of EVs in enhancing the infectivity of SARS-CoV-2, which is opposing to the strategy to employ EVs, especially ACE2-EV, as a therapeutic option for SARS-CoV-2 infection.
- Subjects
SARS-CoV-2; EXTRACELLULAR vesicles; VIRUS inactivation
- Publication
Journal of Extracellular Vesicles, 2022, Vol 11, Issue 5, p1
- ISSN
2001-3078
- Publication type
Article
- DOI
10.1002/jev2.12231