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- Title
Speciation Analysis of Mercury in Sediments Using HPLC Hyphenated to Vapour Generation Atomic Fluorescence Spectrometry Following Microwave-Assisted Extraction.
- Authors
Geng Leng; Li Feng; Shao-Bo Li; Ping Yang; De-Zhong Dan
- Abstract
A method using high performance liquid chromatography (HPLC) coupled with vapour generation atomic fluorescence spectrometry (VGAFS) has been developed for the separation and determination of mercury species in sediment samples. The method is based on the extraction of samples using 0.1% (v/v) 2-mercaptoethanol as the only extractant. The separation and determination of mercury species was achieved by direct injection of the extractant to the HPLC-VGAFS system. Extraction conditions were optimized, and extraction efficiency was validated against an established method, with relative standard deviation (RSD) below 8%. Three mercury species including inorganic mercury (Hg2+), methylmercury (MeHg+), and ethylmercury (EtHg+) were baseline separated using reversed-phase C18 columns with a mobile phase of 3% (v/v) acetonitriie containing 60 mM ammonium acetate-acetic acid (pH 4.5) and 0.1% (v/v) 2-mercaptoethanol pumped at 2.0 mL/min-1. At optimized conditions, the limit of detection was 0.58 ng/g for MeHg+, 1.1 ng/g for EtHg+ and 0.48 ng/g for Hg2+. The average recoveries were 96.2% (MeHg+), 88.7% (EtHg+) and 95.8% (Hg+) by spiking three concentration levels of mercury species into samples. The developed method was validated by the determination of certified reference materials and was further applied in analyses of sediment samples from the Sichuan area in China.
- Subjects
CHEMICAL speciation; MERCURY spectra; HIGH performance liquid chromatography; AMMONIUM acetate; FLUORESCENCE spectroscopy; MERCAPTOETHANOL; EXTRACTION (Chemistry)
- Publication
LC-GC Europe, 2013, Vol 26, Issue 5, p250
- ISSN
1471-6577
- Publication type
Article