We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of diosgenin in the inhibition of gastric cancer cell proliferation and its mechanism in combination with TRAIL to induce apoptosis.
- Authors
Qingwei Zhang; Sheng Zheng; Xuelian Bai; Chuntian Ma; Yuqian Qi
- Abstract
Clinical observations suggest that identifying novel curative and preventive approaches by targeting the tumor cells without affecting the normal cells is appreciable. Diosgenin (DG) is a natural sapogenin that has been reported of having pro-apoptotic and anti-cancer properties against various neoplasia. Hence, the present study investigated the effect of combined DG/TNF-related apoptosis-inducing ligand (TRAIL) on gastric cancer cell lines (BGC-823) in vitro. Cell viability and IC50 for DG and TRAIL alone or in combination were determined using MTT. The apoptosis rate was assessed by ELISA cell death assay and Caspase 8 activity assays. The gene expression evaluation of candidate genes, including survivin, Bcl-2, XIAP, c-IAP1, c-IAP2, and c-FLIP, were accomplished before and after the treatment by quantitative real-time PCR. Our results demonstrated that DG synergistically enhanced the cytotoxic effects of TRAIL (p < 0.01). DG could exaggerate cell apoptosis through TRAIL-induced apoptosis and amplify the Caspase 8 activity. These results were confirmed by decreasing anti-apoptotic genes' expression levels. Overall, our findings shed light on a novel strategy of TRAIL-induced apoptosis in combination with DG for the treatment of gastric cancer.
- Subjects
CANCER cell proliferation; STOMACH cancer; DIOSGENIN; CELL death; APOPTOSIS; TRAIL protein; CANCER cells
- Publication
ScienceAsia, 2022, Vol 48, Issue 6, p827
- ISSN
1513-1874
- Publication type
Article
- DOI
10.2306/scienceasia1513-1874.2022.123