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- Title
Improving the Yield and Quality of Daptomycin in Streptomyces roseosporus by Multilevel Metabolic Engineering.
- Authors
Lyu, Zhong-Yuan; Bu, Qing-Ting; Fang, Jiao-Le; Zhu, Chen-Yang; Xu, Wei-Feng; Ma, Lie; Gao, Wen-Li; Chen, Xin-Ai; Li, Yong-Quan
- Abstract
Finally, we further increased daptomycin to 105 mg/l with an increase of 40% by introducing an extra copy of daptomycin BGC, which implied that multicopy of BGC can efficiently enhance the biosynthesis of daptomycin (Figure 3A). However, co-overexpression of other related genes, such as I dptJ i , I orf3245 i , and I orf3243 i , made daptomycin production decrease by 26% (Figure 2B) and this may be due to the excessive accumulation of some intermediates, which affect the further synthesis of L-Kyn ([11]) or are toxic to the cell itself, thereby affecting the accumulation of daptomycin. A previous study showed that daptomycin BGC with only 65 kb length was enough to be heterologously expressed in daptomycin non-producing strain I S. coelicolor i M511 and successfully led to the production of 28.9 mg/l daptomycin ([6]).
- Subjects
DAPTOMYCIN; STREPTOMYCES; ANTIBIOTIC synthesis; LIPOPEPTIDE antibiotics; NONRIBOSOMAL peptide synthetases; GENE clusters; POLYKETIDE synthases; ANTIBIOTICS
- Publication
Frontiers in Microbiology, 2022, Vol 13, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2022.872397