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- Title
Cost-effective diagnosis of male oxidative stress using the nitroblue tetrazolium test: useful application for the developing world.
- Authors
Amarasekara, D. S.; Wijerathna, S.; Fernando, C.; Udagama, P. V.
- Abstract
Seminal oxidative stress plays an important role in male factor infertility ( MFI), worldwide. A study was thus undertaken for the first time to establish seminal reactive oxygen species ( ROS) as a clinical marker of MFI in a cohort of Sri Lankan males. The nitro blue tetrazolium ( NBT) assay for ROS estimation and modified Endtz test for detecting leucocytes were carried out on semen samples ( N = 102) of subfertile males. Age-matched individuals ( N = 30) with proven past paternity served as controls. Significantly higher ROS production was evident in individuals with asthenozoospermia and unexplained infertility (Mann-Whitney U-test, P = 0.000), than in the fertile and the other subfertile groups tested. Receiver operating characteristic plot analysis established cut-off points of 40.57 and 42.02 μg formazan/107 spermatozoa for ROS to distinguish fertile males from asthenozoospermics (71.4% sensitivity: 70% specificity; AUC = 0.82), and from unexplained infertile males (74.1 % sensitivity: 73.3% specificity; AUC = 0.85) respectively. As ROS appear to be a potential marker of male infertility, it is imperative to validate this test as a simple, cost-effective hence a widely accessible diagnostic tool to be included in MFI investigations in the developing world.
- Subjects
COST effectiveness; OXIDATIVE stress; NITROBLUE tetrazolium; MALE infertility; REACTIVE oxygen species; SPERMATOZOA physiology
- Publication
Andrologia, 2014, Vol 46, Issue 2, p73
- ISSN
0303-4569
- Publication type
Article
- DOI
10.1111/and.12043