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- Title
Effect of Different Substrates and Carbon and Nitrogen Sources on Growth and Shelf Life of Trichoderma pseudokoningii.
- Authors
Rajput, Abdul Qayoom; Khanzada, Muhammad Ali; Shahzad, Saleem
- Abstract
Trichoderma pseudokoningii is a hyperparasite on hyphae of many plant pathogens. However, no reports on its mass production are available. Nine different organic substrates viz., rice grains, sorghum grains, wheat grains, millet grains, wheat straw, rice husk, cow dung, sawdust and poultry manure were therefore evaluated for mass multiplication of T. pseudokoningii. Grains, especially sorghum grains were found more suitable for growth and sporulation than wheat straw, rice husk and cow dung. Sawdust and poultry manure were least effective. Sucrose and dextrose were found to be the most suitable carbon sources, whereas ammonium nitrate was found to be the best nitrogen source for growth and sporulation of T. pseudokoningii on Czapek's Agar plates. Amendment of selected C and N sources to organic substrates resulted in greater growth and conidia production by T. pseudokoningii on wheat straw, rice husk and millet grains but not on sorghum and rice grains. During studies on shelf life, populations of T. pseudokoningii on different substrates attained the peck at 60-75 d interval and declined gradually thereafter. However, even after 330 d, the populations were greater than the population at 0-day. At 345-360 d interval, populations were less than the initial populations at 0 d. © 2014 Friends Science Publishers
- Subjects
BIOCHEMICAL substrates; PLANT growth; TRICHODERMA; PHYTOPATHOGENIC microorganisms; ORGANIC compounds; WHEAT straw
- Publication
International Journal of Agriculture & Biology, 2014, Vol 16, Issue 5, p893
- ISSN
1560-8530
- Publication type
Article