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- Title
N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural Stem/Progenitor Cells Induced by Aβ<sub>1–42</sub> via the CB2 Receptor.
- Authors
Li, Wen-Qing; Wang, Ze-jian; Liu, Sha; Hu, Yue; Yin, Ming; Lu, Yang
- Abstract
Alzheimer’s disease, one of the neurodegenerative diseases, shows the progressive senescence of neural progenitor/stem cells. N-Stearoyl-L-tyrosine (NsTyr) showed neuroprotective effect against chronic brain ischemia in previous reports. In the present study, we find the antisenescent effects of NsTyr-2K in NSPCs induced by Aβ1–42 in vitro. Cell viability of NSPCs was evaluated by CCK8 assay; SA-β-gal staining was used to evaluate senescence of NSPCs. CB receptors were detected by immunohistochemistry in NSPCs. AM251 or AM630 was used to offset the anti-senescence effects afforded by NsTyr-2K. The positive rate of SA-β-gal staining was significantly increased in NSPCs after incubation with Aβ1–42 for 9 days. CB receptors were found on the surface of NSPCs. The expression level of CB1 receptors was significantly decreased in NSPCs after incubation with Aβ1–42. This phenomenon was reversed dose-dependently by NsTyr-2K. NsTyr-2K attenuated Aβ1–42 induced NSPCs senescence dose-dependently, and its antisenescence effect was completely abolished by AM630. Aβ1–42 dose-dependently increased the prosenescence molecules p16 and Rb. Their expression was inhibited by NsTyr-2K dose-dependently and blocked by AM630 in NSPCs. These results suggest that NsTyr-2K can alleviate the senescence of NSPCs induced by Aβ1–42 via CB2 receptor.
- Subjects
NEURAL stem cells; CANNABINOID receptors; ALZHEIMER'S patients; NEURODEGENERATION; NEUROPROTECTIVE agents; CEREBRAL ischemia; PREVENTION
- Publication
Thrombosis, 2016, p1
- ISSN
2090-1488
- Publication type
Article
- DOI
10.1155/2016/7419389