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- Title
Structural variation in the O-specific polysaccharides of Klebsiella pneumoniae serotype O1 and O8 lipopolysaccharide: evidence for clonal diversity in rfb genes.
- Authors
Kelly, Robert F.; Severn, Wayne B.; Richards, James C.; Perry, Malcolm B.; MacLean, Leann L.; Tomás, Juan M.; Merino, Susana; Whitfield, Chris
- Abstract
The O-polysaccharide fraction of the lipopolysaccharide from <em>Klebsiella pneumoniae</em> serotype 08 was found to comprise two galactose-containing homopolymers. Structural analysis, using chemical and high-field nuclear magnetic resonance (NMR) techniques, established that the <em>K. pneumoniae</em> 08 polysaccharides are composed of the linear, disaccharide repeating units. ... <em>K. pneumoniae</em> O8 mutant RFK-1 was isolated by resistance to phage KO1-2; strain RFK-1 expressed only D-galactan I-OAc. The 1H- and 13C-NMR resonances from this O-polysaccharide indicate that all of the <em>O</em>-acetyl groups within the <em>K. pneumoniae</em> 08 polysaccharide are carried on D-galactan I and <em>O</em>-acetylation occurs only on the β-D-galactofuranose residues; 60% of the available β-D-galactofuranose residues are non-acetylated. The <em>O</em>-acetylation of the remaining residues is equally distributed between the O-2 and O-6 positions. The carbohydrate backbone structures in the 08 polysaccharide are identical to D-galactan I and II expressed by <em>K. pneumoniae</em> O1, accounting for the antigenic cross-reaction between strains belonging to serotypes O1 and 08. However, the O1 polysaccharides are not acetylated and the <em>O</em>-acetyl groups present in the <em>K. pneumoniae</em> serotype 08 polysaccharides provide a structural basis for their recognition as distinct serotypes. The <em>rfb</em> (O-polysaccharide biosynthesis) gene cluster of <em>K. pneumoniae</em> serotype O1 determines the synthesis of D-galactan I. <em>rfb</em>KpO1-specific gene probes were used to examine conservation in the <em>rfb</em> gene clusters of other <em>K. pneumoniae</em> serotypes which produce D-galactan I. Six O1 strains were examined and all showed hybridization with <em>rfb</em>KpO1 probes under conditions of high stringency. Three serotype O2 strains produce D-galactan I and these strains also contained DNA...
- Subjects
POLYSACCHARIDES; KLEBSIELLA pneumoniae; ENDOTOXINS; GALACTOSE; POLYMERS; GENES
- Publication
Molecular Microbiology, 1993, Vol 10, Issue 3, p615
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1993.tb00933.x