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- Title
A Bumpy Ride on the Diagnostic Bench of Massive Parallel Sequencing, the Case of the Mitochondrial Genome.
- Authors
Vancampenhout, Kim; Caljon, Ben; Spits, Claudia; Stouffs, Katrien; Jonckheere, An; De Meirleir, Linda; Lissens, Willy; Vanlander, Arnaud; Smet, Joél; De Paepe, Boel; Van Coster, Rudy; Seneca, Sara
- Abstract
The advent of massive parallel sequencing (MPS) has revolutionized the field of human molecular genetics, including the diagnostic study of mitochondrial (mt) DNA dysfunction. The analysis of the complete mitochondrial genome using MPS platforms is now common and will soon outrun conventional sequencing. However, the development of a robust and reliable protocol is rather challenging. A previous pilot study for the re-sequencing of human mtDNA revealed an uneven coverage, affecting predominantly part of the plus strand. In an attempt to address this problem, we undertook a comparative study of standard and modified protocols for the Ion Torrent PGM system. We could not improve strand representation by altering the recommended shearing methodology of the standard workflow or omitting the DNA polymerase amplification step from the library construction process. However, we were able to associate coverage bias of the plus strand with a specific sequence motif. Additionally, we compared coverage and variant calling across technologies. The same samples were also sequenced on a MiSeq device which showed that coverage and heteroplasmic variant calling were much improved.
- Subjects
MITOCHONDRIAL DNA abnormalities; NUCLEOTIDE sequencing; MOLECULAR genetics; GENOMES; DNA polymerases; GENE amplification
- Publication
PLoS ONE, 2014, Vol 9, Issue 11, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0112950