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- Title
Imiquimod Induces Apoptosis of Squamous Cell Carcinoma (SCC) Cells via Regulation of A20.
- Authors
Sohn, Kyung-Cheol; Li, Zheng Jun; Choi, Dae-Kyoung; Zhang, Tiejun; Lim, Jae Woo; Chang, In-Kyu; Hur, Gang Min; Im, Myung; Lee, Young; Seo, Young-Joon; Lee, Jeung-Hoon; Kim, Chang Deok
- Abstract
Imiquimod, a nucleoside analogue of the imidazoquinoline family, is being used to treat various cutaneous cancers including squamous cell carcinoma (SCC). Imiquimod activates anti-tumor immunity via Toll-like receptor 7 (TLR7) in macrophage and other immune cells. Imiquimod can also affect tumor cells directly, regardless of its impact on immune system. In this study, we demonstrated that imiquimod induced apoptosis of SCC cells (SCC12) and A20 was involved in this process. When A20 was overexpressed, imiquimod-induced apoptosis was markedly inhibited. Conversely, knockdown of A20 potentiated imiquimod-induced apoptosis. Interestingly, A20 counteracted activation of c-Jun N-terminal kinase (JNK), suggesting that A20-regulated JNK activity was possible mechanism underlying imiquimod-induced apoptosis of SCC12 cells. Finally, imiquimod-induced apoptosis of SCC12 cells was taken place in a TLR7-independent manner. Our data provide new insight into the mechanism underlying imiquimod effect in cutaneous cancer treatment.
- Subjects
CANCER treatment; SQUAMOUS cell carcinoma; APOPTOSIS; CANCER cells; NUCLEOSIDES; TOLL-like receptors; C-Jun N-terminal kinases
- Publication
PLoS ONE, 2014, Vol 9, Issue 4, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0095337