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- Title
Cryo-techniques applied to stratum corneum with description of a new sample holder for cryo-scanning electron microscopy of freeze-fractured samples.
- Authors
Hallegot; Minondo; Fiat; Hallegot
- Abstract
Stratum corneum structure greatly differs from that of the living epidermis and specific sample cryo-preparation techniques have to be used. Practical aspects of these cryo-techniques applied to stratum corneum are discussed. Emphasis is placed on scanning electron microscopy of cryo-fixed samples. A new sample holder designed for cryo-scanning electron microscopy of freeze-fractured stratum corneum is described.
- Subjects
ELECTRON microscopy; CRYOELECTRONICS; FREEZE fracturing
- Publication
Journal of Microscopy, 1999, Vol 196, Issue 1, p35
- ISSN
0022-2720
- Publication type
Article
- DOI
10.1046/j.1365-2818.1999.00586.x