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- Title
Checkpoints in Adenoviral Production: Cross-Contamination and E1A.
- Authors
Haeussler, Dagmar J.; Evangelista, Alicia M.; Burgoyne, Joseph R.; Cohen, Richard A.; Bachschmid, Markus M.; Pimental, David R.
- Abstract
Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.
- Subjects
ADENOVIRUSES; GENE expression; MAMMALIAN cell cycle; GENETIC transcription; GENE amplification; VIRAL genes; CELL physiology; DATA analysis
- Publication
PLoS ONE, 2011, Vol 6, Issue 8, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0023160