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- Title
Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study.
- Authors
Chen, Mark I.; Barr, Ian G.; Koh, Gerald C. H.; Lee, Vernon J.; Lee, Caroline P. S.; Shaw, Robert; Lin, Cui; Yap, Jonathan; Cook, Alex R.; Boon Huan Tan; Jin Phang Loh; Barkham, Timothy; Vincent T. K. Chow; Raymond T. P. Lin; Yee-Sin Leo
- Abstract
Background: We describe the serological response following H1N1-2009 influenza A infections confirmed by reversetranscriptase polymerase chain reaction (RT-PCR). Methodology and Principal Findings: The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1- 2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively. Conclusions and Significance: Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.
- Subjects
H1N1 influenza; SEROLOGY; REVERSE transcriptase polymerase chain reaction; BLOOD agglutination; VACCINES; IMMUNOGLOBULINS; ANTIBODY titer; CROSS reactions (Immunology); SCIENTIFIC observation; PHYSIOLOGY
- Publication
PLoS ONE, 2010, Vol 5, Issue 8, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0012474