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- Title
Cardioprotective effects of rat adipose-derived stem cells differ under normoxic/physioxic conditions and are associated with paracrine factor secretion.
- Authors
Lin, Yuanyuan; Li, Xuewen; Fan, Chunhui; Yang, Fan; Hao, Dajie; Ge, Wenjia; Gao, Yuping
- Abstract
Adipose tissue-derived stem cells (ASCs) are beneficial for myocardial regeneration. The physiological oxygen content of human organs is estimated to range between 1 and 11%. However, in the majority of previous in vitro studies with cultured ASCs, the O2 concentration was artificially set to 21%. The present study aimed to compare the protective effects of rat ASCs on neonatal rat ventricular myocytes (NRVMs) under normoxic (21% O2) and physioxic (5% O2) conditions. Rat NRVMs cultured under normoxia or physioxia were treated with H2O2 or left untreated, and further co-cultured with ASCs in 21% or 5% O2. The apoptosis of NRVMs was evaluated by Annexin V staining and quantitating the protein levels of Bcl-2 and Bax by western blotting. The oxidative stress of NRVMs was determined by a glutathione/oxidized glutathione assay kit. The concentrations of secreted vascular endothelium growth factor (VEGF), insulin like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) in the culture medium were quantified by enzyme-linked immunosorbent assay. Under both normoxia and physioxia, co-culture with ASCs protected H2O2-exposed NRVMs from apoptosis and significantly alleviated the oxidative stress in NRVMs. The protective effects of ASCs were associated with increased secretion of VEGF, IGF-1 and bFGF. ASCs cultured in 5% O2 exhibited certain cardioprotective effects against H2O2 stress. The results of the present study suggested that O2 concentrations influenced the cardioprotective effects of ASCs. VEGF, IGF-1 and bFGF may serve a role in the myocardial regeneration mediated by transplanted ASCs.
- Publication
International Journal of Molecular Medicine, 2020, Vol 45, Issue 5, p1591
- ISSN
1107-3756
- Publication type
Article
- DOI
10.3892/ijmm.2020.4524