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- Title
De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing.
- Authors
Kakumani, Pavan Kumar; Shukla, Rohit; Todur, Vivek N.; Malhotra, Pawan; Mukherjee, Sunil K.; Bhatnagar, Raj K.
- Abstract
Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.
- Publication
Biology Direct, 2015, Vol 10, Issue 1, p44
- ISSN
1745-6150
- Publication type
Article
- DOI
10.1186/s13062-015-0072-7