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- Title
Selective Targeting of Adenoviral Vectors to Neural Precursor Cells in the Hippocampus of Adult Mice: New Prospects for In Situ Gene Therapy.
- Authors
Schmidt, Anke; Haas, Stefan J.-P.; Hildebrandt, Steve; Scheibe, Johanna; Eckhoff, Birthe; Racek, Tomáš; Kempermann, Gerd; Wree, Andreas; Pützer, Brigitte M.
- Abstract
The adult brain contains neural precursor cells (NPC) that are attracted to brain lesions, such as areas of neurodegeneration, ischemia, and cancer. This suggests that NPC engineered to promote lineage-specific differentiation or to express therapeutic genes might become a valuable tool for restorative cell therapy and for targeting therapeutic genes to diseased brain regions. Here we report the identification of NPC-specific ligands from phage display peptide libraries and show their potential to selectively direct adenovirusmediated gene transfer to NPC in adult mice. Identified peptides mediated specific virus binding and internalization to cultured neurospheres. Importantly, peptide-mediated adenoviral vector infection was restricted to precursor cells in the hippocampal dentate gyrus of pNestingreen fluorescent protein transgenic or C57BL/6 mice. Our approach represents a novel method for specific manipulation of NPC in the adult brain and may have major implications for the use of precursor cells as therapeutic delivery vehicles in the central nervous system.
- Subjects
CELL differentiation; GENE therapy; ISCHEMIA; LIGANDS (Biochemistry); PEPTIDES; GENETIC transformation
- Publication
Stem Cells, 2007, Vol 25, Issue 11, p2910
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2007-0238