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- Title
Kinetics of Theta-Positive and Theta-Negative Lymphocytes in Thymus-Deprived and Normal Mice.
- Authors
Röpke, C.
- Abstract
Life spans and proliferative kinetics of theta-positive (θ+) and theta-negative ((θ-) cells were evaluated in normal, neonatally thymectomized (NeoTx) and sham-thymectomized (ShamTx) Balb/c mice. This was done by exposing cell suspensions, incubated with anti-θ antiserum + complement, to a dye exclusion test followed by fixation and autoradiography. Results from normal mice given [³H]thymidine injections 5 weeks before being killed indicated that long-lived θ+ and θ- cells constituted about equal percentages of the respective populations in peripheral lymphoid tissues. Long-lived θ+ cells constituted a relatively high percentage of θ+ cells in the bone marrow, whereas a minority of the θ- lymphocytes were long-lived. Results from NeoTx and ShamTx mice, given intensive injections of [³H]thymidine, showed that the θ- cells of the mesenteric nodes were renewed at comparable rates in both groups of mice, whereas the θ+ cells were more rapidly renewed in NeoTx mice than in shamoperated littermates. In the bone marrow, the majority of both θ- and θ+ cells were very rapidly renewed, indicating a production of these cells in the bone marrow of ShamTx mice and also in NeoTx mice, in which the number of bone-marrow θ+ cells was significantly higher than in ShamTx littermates.
- Subjects
THY-1 antigen; AUTORADIOGRAPHY; THYMIDINE; LYMPHOID tissue; LABORATORY mice; BONE marrow
- Publication
Scandinavian Journal of Immunology, 1977, Vol 6, Issue 4, p291
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1977.tb00396.x