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- Title
Point-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening †.
- Authors
Demuru, Silvia; Chai-Gao, Hui; Shynkarenko, Yevhen; Hermann, Nicola; Boia, Patricia-Daiana; Cristofolini, Peter; Petkus, Bradley; Generelli, Silvia; Paoletti, Samantha; Cattaneo, Stefano; Burr, Loïc
- Abstract
With the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergen-specific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 μL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for large-scale research in allergies.
- Subjects
IMMUNOGLOBULIN E; MICROFLUIDICS; MEDICAL screening; FLUORESCENCE; HOUSE dust mites; POINT-of-care testing; DOG shows
- Publication
Sensors (14248220), 2024, Vol 24, Issue 11, p3280
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s24113280