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- Title
Paracrine Regulation of Insulin-Like Growth Factor I (IGF-I) and IGF-II on Prostaglandins F<sub>2α</sub> and E<sub>2</sub> Synthesis by Human Corpus Luteum in Vitro: A Possible Balance of Luteotropic and Luteolytic Effects.
- Authors
APA, ROSANNA; MICELI, FIORELLA; PIERRO, EMILIA; MINICI, FRANCESCA; NAVARRA, PIERLUIGI; CARUSO, ALESSANDRO; NAPOLITANO, MADDALENA; MANCUSO, SALVATORE; LANZONE, ANTONIO
- Abstract
The existence of a complete intraovarian insulin-like growth factor (IGF) system replete with ligands, receptors, and binding proteins has been demonstrated as well as the ability of IGF-I to positively affect steroidogenesis in human granulosa cells. Furthermore, we recently showed that IGF-I and IGF-II stimulate progesterone secretion by human luteal cells. As the PGs, PGE2 and PGF2α, are classically known to have luteotropic and luteolytic effects, we wanted to determine whether the IGFs could affect the human luteal phase by influencing the PG system. For this reason, human luteal cells were cultured for different times (12, 24, and 48 h) with IGF-I, IGF-II (10-100 ng/mL), and GH (100 ng/mL), and both PGs were assayed in the medium culture. We found that both IGF-I and IGF-II were able to stimulate PGE2 synthesis in a time- and dose-dependent way, whereas they both inhibited PGF2α production. GH, too, significantly reduced PGF2α synthesis; this effect was IGF-I mediated because it was reverted by increasing dilutions of an anti-IGF-I antibody. On the contrary, no GH effect was observed on PGE2 production. In conclusion, based on these data and on our previous results, we speculate that IGFs could influence luteal steroidogenesis through PG system.
- Publication
Journal of Clinical Endocrinology & Metabolism, 1999, Vol 84, Issue 7, p2507
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jcem.84.7.5852