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- Title
Biostimulative effects of ND:YAG q-switch dye on normal human fibroblast cultures: Study of a new chemosensitizing agent for the ND:YAG laser.
- Authors
Castro, Dan J.; Ward, Paul H.; Saxton, Romaine E.; Castro, Donna J.; Fetterman, Harold R.
- Abstract
Kodak Q-switch II® is a new chemical with an absorption maxima at 1,051 nm, designed to be used as an Nd:YAG dye laser. The potential for this dye as a new chemosensitizing agent in the treatment of connective tissue diseases and wound healing with low energy Nd:YAG laser was examined. Two normal fibroblast cell lines were tested for sensitivity to various levels of this dye in vitro. These cells were exposed to Q-switch II dye at concentrations of 0.01, 0.1, 1, 10, 50, and 100 μg/ml for 1 and 24 hours. Cell viability was assessed by the trypan blue exclusion test. Cell duplication and DNA synthesis were measured by the incorporation of [3H]-thymidine at 6 and 24 hours postexposure to Q-switch II dye. At concentrations up to 10 μg/ml, both cell lines tested showed no changes in cell viability. However, at concentrations equal or higher than 50 μg/ml, more than 40% of the fibroblasts incorporated trypan blue after 24 hours of exposure to this dye, indicating significant cell destruction. The results indicate that Q-switch II dye is nontoxic to normal human fibroblast cultures and showed significant biostimulative effects on cell duplication at concentrations equal to or lower than 10 μg/ml. Further studies will be required to determine the usefulness of Q-switch EC dye as a new photochemosensitizing agent for potential biostimulation of wound healing and/or treatment of connective tissue diseases with the Nd:YAG laser (near infrared, 1,060 nm) at 'nonthermal' levels of energies.
- Publication
Laryngoscope, 1987, Vol 97, Issue 12, p1454
- ISSN
0023-852X
- Publication type
Article
- DOI
10.1288/00005537-198712000-00014