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- Title
MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI-BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL.
- Authors
Garcia TELES, Carolina Bioni; MEDEIROS, Jansen Fernandes; dos SANTOS, Ana Paula de Azevedo; de FREITAS, Luís Antônio Rodrigues; KATSURAGAWA, Tony Hiroshi; CANTANHÊDE, Lilian Motta; Mattos FERREIRA, Ricardo de Godoi; Aranha CAMARGO, Luís Marcelo
- Abstract
In this study, Leishmania species were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR, the DNA of Leishmania was detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70 gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%), L. (V.) shawi (28.1%), L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis (3.1%). This is the first report of L. (V.) shawi and L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting.
- Subjects
UNITED States; CUTANEOUS leishmaniasis; POLYMERASE chain reaction; EPIDEMIOLOGICAL research; PUBLIC health; RESTRICTION fragment length polymorphisms; LEISHMANIA
- Publication
Revista do Instituto de Medicina Tropical de São Paulo, 2015, Vol 57, Issue 4, p343
- ISSN
0036-4665
- Publication type
Article
- DOI
10.1590/S0036-46652015000400012