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- Title
High-throughput in vivo vertebrate screening.
- Authors
Pardo-Martin, Carlos; Tsung-Yao Chang; Bryan Kyo Koo; Gilleland, Cody L.; Wasserman, Steven C.; Yanik, Mehmet Fatih
- Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
- Subjects
IMAGING systems in biology; CONFOCAL microscopy; AXONS; FEMTOSECOND lasers; ZEBRA danio; NERVOUS system regeneration
- Publication
Nature Methods, 2010, Vol 7, Issue 8, p634
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1481