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- Title
Polysaccharides isolated from Morinda officinalis How roots inhibits cyclophosphamide-induced leukopenia in mice.
- Authors
Yaxin Zhao; Meng Wang; Yang Li; Wanting Dong
- Abstract
Purpose: To investigate the optimum parameters for extracting polysaccharides from Morinda officinalis How (MOP), and explore their inhibitory effects on leukopenia in mice. Methods: Orthogonal design was performed to investigate the optimum parameters for extracting MOP. A leukopenia mouse model was established by injection of cyclophosphamide (CTX) for three days. Thereafter, MOP (100, 200 and 400 mg/kg) was administered orally for 10 days. Furthermore, blood cells (leukocytes, neutrophil, lymphocyte and mononuclear cell) were analyzed, while serum IL-3 and IL-6 were determined by ELISA. The thymus and spleen of the mice were separated and weighed to determine viscera indices. Results: Orthogonal design showed that the influence order of the four factors was extraction times (C) > ratio of water to raw material (RWM, D) > extraction time (B) > extraction temperature (A). The optimum extraction parameters for MOP were: extraction temperature (80 °C), extraction duration (2 h), no. of extractions (3), and ratio of water to raw material (30 mL/g). Furthermore, the results indicate that MOP (100, 200 and 400 mg/kg) elevated the levels of leukocyte (p < 0.01), neutrophil (p < 0.01), lymphocyte (p < 0.01) and mononuclear cell (p < 0.01) in leukopenia mice. Besides, MOP (100, 200 and 400 mg/kg) also increased thymus (p < 0.01) and spleen (p < 0.05) indices and serum levels of IL-3 (p < 0.05) and IL-6 (p < 0.01). Conclusion: Orthogonal design is a good strategy for optimizing extraction parameters of MOP. Furthermore, MOP stimulated synthesis of leukocytes in CTX-induced leukopenia in mice. Thus, MOP is a potential adjunct for the treatment of tumors/cancers.
- Subjects
POLYSACCHARIDES; MORINDA; CYCLOPHOSPHAMIDE; BLOOD cells; NEUTROPHILS; LYMPHOCYTES
- Publication
Tropical Journal of Pharmaceutical Research, 2017, Vol 16, Issue 9, p2155
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v16i9.16