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- Title
Somatic embryogenesis and plant regeneration from suspension cultures of Sapindus trifoliatus.
- Authors
Singh, Madhulika; Asthana, Pooja; Rai, Manoj K.; Jaiswal, Uma
- Abstract
A procedure for plant regeneration from cell suspension cultures through somatic embryogenesis is described for Sapindus trifoliatus, a commercially and medicinally important tree. Callus was induced from leaf disc on agar-solidified MS medium with 5.0 mg l−1 2, 4-D and 0.01 mg l−1 Kin. Embryogenic cell suspension cultures were established by placing leaf-derived friable calli in PGR-free full-strength MS liquid medium with 3% sucrose. The growth of cell suspension culture was significantly affected by the strength of the MS mineral solution and L-glutamine. Plating of the suspension on semisolid MS medium resulted in the formation of globular structures. These embryogenic globular structures differentiated into secondary globular structures or somatic embryos on a semisolid MS medium. The differentiation of globular structures and different stages of somatic embryos (from globular to cotyledonary) was enhanced by the addition of 200 mg l−1 L-glutamine in the medium. Sucrose at relatively high concentrations (5%) or ABA (0.01 mg l−1) promoted somatic embryo maturation. The highest percentage (about 90%) of germination of somatic embryo and plantlet conversion was achieved on a half-strength MS medium containing 2% sucrose. The plants were hardened and established in soil with a 90% survival rateKey message: The present study demonstrates the establishment of suspension culture from leaf-derived callus in Sapindus trifoliatus and plant regeneration via somatic embryogenesis
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 157, Issue 2, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-024-02760-2