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- Title
Transmembrane routes of cationic liposome-mediated gene delivery using human throat epidermis cancer cells.
- Authors
Cui, Shaohui; Wang, Bing; Zhao, Yinan; Chen, Huiying; Ding, Huiqin; Zhi, Defu; Zhang, Shubiao
- Abstract
For studying the mechanism of cationic liposome-mediated transmembrane routes for gene delivery, various inhibitors of endocytosis were used to treat human throat epidermis cancer cells, Hep-2, before transfection with Lipofectamine 2000/pGFP-N2 or Lipofectamine 2000/pGL3. To eliminate the effect of inhibitor toxicity on transfection, the RLU/survival rate was used to represent the transfection efficiency. Chlorpromazine and wortmannin, clathrin inhibitors, decreased transfection efficiency by 44 % (100 μM) and 31 % (100 nM), respectively. At the same time, genistein, a caveolin inhibitor, decreased it by 30 % (200 μM). Thus combined transmembrane routes through the clathrin and caveolae-mediated pathways were major mechanisms of cell uptake for the cationic liposome-mediated gene delivery. After entering the cells, microtubules played an important role on gene delivery as vinblastine, a microtubulin inhibitor, could reduce transfection efficiency by 41 % (200 nM).
- Subjects
CANCER cells; EPIDERMIS; THROAT; LIPOSOMES; BASIC proteins; MEMBRANE proteins; ENZYME inhibitors; GENE transfection; CANCER
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 1, p1
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1325-0