We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of a chloroplast DNA marker for monitoring of transgene introgression in Brassica napus L.
- Authors
Woo, Hee-Jong; Lim, Myung-Ho; Shin, Kong-Sik; Martins, Bianca; Lee, Bum-Kyu; Cho, Hyun-Suk; Mallory-Smith, Carol
- Abstract
Chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in Brassicaceae and for differentiation between its species. Combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (GM) plants through hybridization studies. To develop chloroplast DNA markers for monitoring of transgene introgression in Brassica napus L., we searched for sequence variations in the chloroplast (cp) genome, and developed a simple cpDNA marker that is reliable, time-saving, and easily discriminates among 4 species ( B. napus, B. rapa, Raphanus sativus, and Sinapis alba) based on PCR-product length polymorphism. This marker will be useful to identify maternal lineages and to estimate transgene movement of GM canola.
- Subjects
CHLOROPLASTS; EXTRACHROMOSOMAL DNA; CHROMATOPHORES; BRASSICA; GENETICS
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 9, p1533
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1236-0