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- Title
Combinational biosynthesis of a fluorescent cyanobacterial holo-α-allophycocyanin in Escherichia coli.
- Authors
Yu Yang; Baosheng Ge; Xiangyu Guan; Weijie Zhang; Song Qin
- Abstract
Allophycocyanin (APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-α subunit (holo-ApcA) was constructed, resulting in over-expression of a fluorescent holo-ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 g l−1, and the expression of holo-HT-ApcA was up to 1 g l−1 broth. The recombinant protein showed similar spectral features to native APC.
- Subjects
BIOCHEMICAL research; CYANOBACTERIA biotechnology; CHEMICAL reactions; BIOSYNTHESIS; BACTERIAL genetics; ESCHERICHIA coli; PHYCOBILIPROTEINS; PHOTOSYNTHETIC pigments; GENE expression; RECOMBINANT proteins; MOLECULAR cloning
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 6, p1001
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9644-2