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- Title
Simultaneous Analysis of Iridoid Glycosides and Anthraquinones in Morinda officinalis Using UPLC-QqQ-MS/MS and UPLC-Q/TOF-MSE.
- Authors
Zhao, Xiangsheng; Wei, Jianhe; Yang, Meihua; Parpinello, Giuseppina Paola; McPhee, Derek J.
- Abstract
<italic>Morinda officinalis</italic> is an important herbal medicine and functional food, and its main constituents include anthraquinone and iridoid glycosides. Quantification of the main compounds is a necessary step to understand the quality and therapeutic properties of <italic>M. officinalis</italic>, but this has not yet been performed based on liquid chromatography/tandem mass spectrometry (LC-MS/MS). Analytes were extracted from <italic>M. officinalis</italic> by reflux method. Ultrahigh-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UPLC-QqQ-MS) using multiple reaction monitoring (MRM) mode was applied for quantification. Fragmentation pathways of deacetyl asperulosidic acid and rubiadin were investigated based on UPLC with quadrupole time-of-flight tandem mass spectrometry (Q/TOF-MS) in the MSE centroid mode. The method showed a good linearity over a wide concentration range (R2 ≥ 0.9930). The limits of quantification of six compounds ranged from 2.6 to 27.57 ng/mL. The intra- and inter-day precisions of the investigated components exhibited an RSD within 4.5% with mean recovery rates of 95.32–99.86%. Contents of selected compounds in <italic>M. officinalis</italic> varied significantly depending on region. The fragmentation pathway of deacetyl asperulosidic and rubiadin was proposed. A selective and sensitive method was developed for determining six target compounds in <italic>M. officinalis</italic> by UPLC-MS/MS. Furthermore, the proposed method will be helpful for quality control and identification main compounds of <italic>M. officinalis</italic>.
- Subjects
MORINDA; IRIDOIDS; GLYCOSIDES; CHINESE medicine; ANTHRAQUINONES
- Publication
Molecules, 2018, Vol 23, Issue 5, p1070
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23051070