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- Title
Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae).
- Authors
Yaping Liang; Xiuwei Li; Zumin Gu; Peiwen Qin; Mingshan Ji
- Abstract
The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by ¹H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin's effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 µmol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 µmol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only.
- Subjects
AMORPHA; EFFECT of poisons on plants; LARVAL behavior; CULEX pipiens; COLUMN chromatography; INFRARED spectra; PLANT extracts
- Publication
Molecules, 2015, Vol 20, Issue 2, p3238
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules20023238