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- Title
Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa.
- Authors
Balaban, Basak; Urman, Bulent; Isiklar, Aycan; Alatas, Cengiz; Mercan, Ramazan; Aksoy, Senai; Nuhoglu, Alp; Balaban, B; Urman, B; Isiklar, A; Alatas, C; Mercan, R; Aksoy, S; Nuhoglu, A
- Abstract
Recent studies indicate a strong paternal influence on embryo development and progression of the embryo to the blastocyst stage. The aim of this study was to compare, during extended culture, the in-vitro development of embryos resulting from intracytoplasmic sperm injection (ICSI) of ejaculated spermatozoa (group 1, n = 347), epididymal (group 2, n = 22) or testicular (group 3, n = 18) spermatozoa from obstructive azoospermic and testicular spermatozoa from non-obstructive azoospermic (group 4, n = 31) subjects. Fertilization and blastocyst formation rates were significantly lower in group 4 (P < 0.05). The incidence of expanded and hatching blastocysts was significantly lower in group 4 (P < 0.05). Overall in 93.2% ejaculate ICSI cycles, blastocysts were transferred on day 5. This was significantly higher than the 62% day 5 transfers in the non-obstructive azoospermic group (P < 0.05). Implantation rate per embryo was significantly higher in the ejaculate ICSI group compared with the other groups (P < 0.05). Clinical pregnancy per transfer was similar between groups; however, significantly fewer multiple pregnancies were encountered in the non-obstructive azoospermic group (P < 0.01). In conclusion, the source of the spermatozoa, most likely to be indicative of the severity of spermatogenic disorder, affects the rate of blastocyst formation and blastocyst implantation. Spermatozoa from non-obstructive azoospermic subjects, when utilized for ICSI, result in embryos that progress to the blastocyst stage at a lower and slower rate and implant less efficiently.
- Subjects
INFERTILITY treatment; COMPARATIVE studies; EJACULATION; EMBRYO transfer; EPIDIDYMIS; FERTILIZATION in vitro; HUMAN reproduction; INFERTILITY; RESEARCH methodology; EVALUATION of medical care; MEDICAL cooperation; PREGNANCY; RESEARCH; SPERMATOZOA; TESTIS; EVALUATION research
- Publication
Human Reproduction, 2001, Vol 16, Issue 1, p125
- ISSN
0268-1161
- Publication type
journal article
- DOI
10.1093/humrep/16.1.125