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- Title
Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3.
- Authors
Wang, Jie; Cheng, Yuqiang; Wang, Longlong; Sun, Aixi; Lin, Zhenyu; Zhu, Wenxian; Wang, Zhaofei; Ma, Jingjiao; Wang, Henan; Yan, Yaxian; Sun, Jianhe
- Abstract
Innate immunity plays an essential role in preventing the invasion of pathogenic microorganisms. However, innate immunity is a double-edged sword, whose excessive activation is detrimental to immune homeostasis and even leads to a "cytokine storm" of the infected host. The host develops a series of negative regulatory mechanisms to balance the immune response. Here, we report a negative regulatory mechanism of chicken innate immunity mediated by miRNA. In the GEO database, we found that miR-126-5p was markedly up-regulated in chickens infected by RNA viruses. Upregulation of miR-126-5p by RNA virus was then further shown via both a cell model and in vivo tests. Overexpression of miR-126-5p significantly inhibited the expression of interferon and inflammatory cytokine-related genes induced by RNA viruses. The opposite result was achieved after the knockdown of miR-126-5p expression. Bioinformatics analysis identified TRAF3 as candidate target gene of miR-126-5p. Experimentally, miR-126-5p can target TRAF3, as shown by the effects of miR-126-5p on the endogenous expression of TRAF3, and by the TRAF3 3'UTR driven luciferase reporter assay. Furthermore, we demonstrated that miR-126-5p negatively regulated innate immunity by blocking the MAVS-TRAF3-TBK1 axis, with a co-expression assay. Overall, our results suggest that miR-126-5p is involved in the negative regulation of chicken innate immunity, which might contribute to maintaining immune balance.
- Subjects
NATURAL immunity; CHICKENS; CYTOKINE release syndrome; RNA viruses; PATHOGENIC microorganisms; DNA microarrays
- Publication
Veterinary Research, 2022, Vol 53, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-022-01098-x