We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
三七总皂苷上调浓缩生长因子释放促进大鼠骨折愈合.
- Authors
王钟庆; 熊贤梅; 张 严; 李世杰; 马立琼; 卢泽声; 高怡加
- Abstract
BACKGROUND: Studies have shown that both Panax notoginseng saponins and concentrated growth factor can promote fracture healing, but there are few studies addressing their combined effects on fracture healing. Panax notoginseng saponins may accelerate fracture healing by promoting the release of concentrated growth factor-related factors over a certain period of time. OBJECTIVE: To study the effect of Panax notoginseng saponins on concentrated growth factor release and fracture healing in rats. METHODS: Eighteen 8-week-old Sprague-Dawley rats were numbered and randomly divided into three groups: Panax notoginseng saponins group, model control group and blank group. Panax notoginseng saponins group was fed with Panax notoginseng saponins for 2 weeks. Model control group was given 2 mL of normal saline for 2 weeks and blank group was fed normally. Concentrated growth factor was obtained by the centrifugation method both from the Panax notoginseng saponins group and model control group. After 1 week of normal feeding, all animals underwent modeling for femoral fracture. The Panax notoginseng saponins group and the model control group were implanted with autologous concentrated growth factor, and then the release concentration of growth factors at different time points (1 hour, 1, 3, 5, 7, 9 and 11 days) were measured by ELISA. Fracture healing was assessed based on postoperative X-ray and hematoxylin-eosin staining of bone tissues. RESULTS AND CONCLUSION: Compared with the model control group, the Panax notoginseng saponins group had higher release concentrations of vascular endothelial growth factor A and transforming growth factor β at 7, 9, and 11 days, Platelet-derived growth factor BB at 5, 9, and 11 days, and basic fibroblast growth factor at 1-11 days (P < 0.01). X-ray examinations indicated that fracture healing in the Panax notoginseng saponins group was better than that in the model control group, and fracture healing in these two groups was better than that in the blank group at 2 months after surgery. Hematoxylin-eosin staining results found that the constituent osteocyte density in the Panax notoginseng saponins group was greater than that in the model control group, and the constituent osteocyte density in these two groups was better than that in the blank group. These findings indicate that Panax notoginseng saponins can increase the concentration of concentrated growth factor-related factors. After intervention with Panax notoginseng saponins, concentrated growth factors are more advantageous in promoting fracture healing in rats.
- Subjects
SAPONINS; FRACTURE healing; FIBROBLAST growth factor 2; VASCULAR endothelial growth factors; TRANSFORMING growth factors; PLATELET-derived growth factor; GROWTH factors
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2024, Vol 28, Issue 11, p1678
- ISSN
2095-4344
- Publication type
Article
- DOI
10.12307/2024.272