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- Title
MiR-200c inhibits autophagy and enhances radiosensitivity in breast cancer cells by targeting UBQLN1.
- Authors
Sun, Quanquan; Liu, Tongxin; Yuan, Yawei; Guo, Zhenli; Xie, Guozhu; Du, Shasha; Lin, Xiaoshan; Xu, Zhixin; Liu, Minfeng; Wang, Wei; Yuan, Quan; Chen, Longhua
- Abstract
Radioresistance is a major challenge during the treatment of breast cancer. A further understanding of the mechanisms of radioresistance could provide strategies to address this challenge. In our study, we compared the expression of miR-200c in four distinct breast cancer cell lines: two representative basal cancer cells (MDA-MB-231 and BT549) vs. two representative luminal cancer cells (MCF-7 and BT474). The results revealed practically lower expression of miR-200c in the two basal cancer cell lines and higher expression of miR-200c in luminal cancer cells compared to the normal breast epithelial cell line MCF-10A. Ectopic expression of miR-200c in MDA-MB-231 cells inhibited irradiation-induced autophagy and sensitized the breast cancer cells to irradiation. We also identified UBQLN1 as a direct functional target of miR-200c involved in irradiation-induced autophagy and radioresistance. In 35 human breast cancer tissue samples, we detected an inverse correlation between the expression of miR-200c vs. UBQLN1 and LC3. These results indicate that the identified miR-200c/UBQLN1-mediated autophagy pathway may help to elucidate radioresistance in human breast cancer and might represent a therapeutic strategy.
- Publication
International Journal of Cancer, 2015, Vol 136, Issue 5, p1003
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.29065