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- Title
Light-Emitting-Diode-Induced Fluorescence from Organic Dyes for Application in Excitation–Emission Fluorescence Spectroscopy for Food System Analysis.
- Authors
Vladev, Veselin; Brazkova, Mariya; Bozhkov, Stefan; Angelova, Galena; Blazheva, Denica; Minkova, Stefka; Nikolova, Krastena; Eftimov, Tinko
- Abstract
An experimental study is presented on the possibility of using the fluorescence from organic dyes as a broadband light source together with a monochromator for applications in excitation–emission matrix (EEM) fluorescence spectroscopy. A high-power single-chip light-emitting diode (LED) was chosen as an excitation source with a central output wavelength at 365 nm to excite a fluorescent solution of Coumarin 1 dye dissolved in ethanol. Two excitation configurations were investigated: direct excitation from the LED and excitation through an optical-fiber-coupled LED. A Czerny–Turner monochromator with a diffraction grating was used for the spectral tuning of the fluorescence. A simple method was investigated for increasing the efficiency of the excitation as well as the fluorescence signal collection by using a diffuse reflector composed of barium sulfate (BaSO4) and polyvinyl alcohol (PVA). As research objects, extra-virgin olive oil (EVOO), Coumarin 6 dye, and Perylene, a polycyclic aromatic hydrocarbon (PAH), were used. The results showed that the light-emitting-diode-induced fluorescence was sufficient to cover the losses on the optical path to the monochromator output, where a detectable signal could be obtained. The obtained results reveal the practical possibility of applying the fluorescence from dyes as a light source for food system analysis by EEM fluorescence spectroscopy.
- Subjects
FLUORESCENCE spectroscopy; ORGANIC dyes; FOOD chemistry; DIFFRACTION gratings; SYSTEM analysis; MONOCHROMATORS; LIGHT emitting diodes
- Publication
Foods, 2024, Vol 13, Issue 9, p1329
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods13091329