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- Title
Diospyrin Modulates Inflammation in Poly I:C-Induced Macrophages via ER Stress-Induced Calcium-CHOP Pathway.
- Authors
Kim, Hyun-Ju; Khan, Inamullah; Shahidullah, Adnan; Halimi, Syed Muhammad Ashhad; Rauf, Abdur; Lee, Ji-Young; Kim, Young-Jin; Kim, Bong-Youn; Park, Wansu
- Abstract
Diospyrin, plant-derived bisnaphthoquinonoid, is known to have anticancer activity. However, pharmacological activity of diospyrin on viral infection is not well known. We investigated effects of diospyrin on macrophages induced by polyinosinic-polycytidylic acid (poly I:C), a mimic of double-stranded viral RNA. Various cytokines, intracellular calcium, nitric oxide (NO), phosphorylated p38 MAPK, and phosphorylated ERK1/2 as well as mRNA expressions of transcription factors were evaluated. Diospyrin significantly reduced NO production, granulocyte-macrophage colony-stimulating factor production, and intracellular calcium release in poly I:C-induced RAW 264.7. The phosphorylation of p38 MAPK and ERK1/2 was also significantly suppressed. Additionally, diospyrin inhibited mRNA levels of nitric oxide synthase 2, C/EBP homologous protein (CHOP), calcium/calmodulin dependent protein kinase II alpha, signal transducers and activators of transcription 1 (STAT1), STAT3, STAT4, Janus kinase 2, first apoptosis signal receptor, c-Jun, and c-Fos in poly I:C-induced RAW 264.7. Taken together, this study represents that diospyrin might have the inhibitory activity against viral inflammation such as excessive production of inflammatory mediators in poly I:C-induced RAW 264.7 via ER stress-induced calcium-CHOP pathway.
- Subjects
GRANULOCYTE-macrophage colony-stimulating factor; NITRIC-oxide synthases; MITOGEN-activated protein kinases; DOUBLE-stranded RNA; TRANSCRIPTION factors; INFLAMMATORY mediators
- Publication
Processes, 2020, Vol 8, Issue 9, p1050
- ISSN
2227-9717
- Publication type
Article
- DOI
10.3390/pr8091050