We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Current Advances in DNA Methylation Analysis Methods.
- Authors
Khodadadi, Ehsan; Fahmideh, Leila; Khodadadi, Ehsaneh; Dao, Sounkalo; Yousefi, Mehdi; Taghizadeh, Sepehr; Asgharzadeh, Mohammad; Yousefi, Bahman; Kafil, Hossein Samadi
- Abstract
DNA methylation is one of the epigenetic changes, which plays a major role in regulating gene expression and, thus, many biological processes and diseases. There are several methods for determining the methylation of DNA samples. However, selecting the most appropriate method for answering biological questions appears to be a challenging task. The primary methods in DNA methylation focused on identifying the state of methylation of the examined genes and determining the total amount of 5-methyl cytosine. The study of DNA methylation at a large scale of genomic levels became possible following the use of microarray hybridization technology. The new generation of sequencing platforms now allows the preparation of genomic maps of DNA methylation at the single-open level. This review includes the majority of methods available to date, introducing the most widely used methods, the bisulfite treatment, biological identification, and chemical cutting along with their advantages and disadvantages. The techniques are then scrutinized according to their robustness, high throughput capabilities, and cost.
- Subjects
DNA analysis; SEQUENCE analysis; MEDICAL technology; DNA methylation; GENE expression; SULFITES; EPIGENOMICS
- Publication
BioMed Research International, 2021, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2021/8827516