We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Eliminating the purple acid phosphatase At PAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization.
- Authors
Robinson, Whitney D.; Carson, Ira; Ying, Sheng; Ellis, Kaya; Plaxton, William C.
- Abstract
Limitation of crop productivity by suboptimal phosphorus ( P) nutrition is a widespread concern. Enhanced crop P-use efficiency could be achieved by improving P remobilization from senescing leaves to developing tissues and seeds. Transcriptomic studies indicate that hundreds of Arabidopsis thaliana genes are up-regulated during leaf senescence, including that encoding the purple acid phosphatase (PAP) At PAP26 (At5g34850)., In this study, biochemical and functional genomic tools were integrated to test the hypothesis that At PAP26 participates in P remobilization during leaf senescence., An eightfold increase in acid phosphatase activity of senescing leaves was correlated with the accumulation of At PAP26 transcripts and immunoreactive At PAP26 polypeptides. Senescing leaves of an atpap26 T- DNA insertion mutant displayed a > 90% decrease in acid phosphatase activity, markedly impaired P remobilization efficiency and delayed senescence. This was paralleled by reduced seed total P concentrations and germination rates., These results demonstrate that At PAP26 loss of function causes dramatic effects that cannot be compensated for by any other PAP isozyme, even though Arabidopsis contains 29 different PAP genes. Our current and earlier studies establish that At PAP26 not only helps to scavenge P from organic P sources when Arabidopsis is cultivated in inorganic orthophosphate ( Pi)-deficient soils, but also has an important P remobilization function during leaf senescence.
- Subjects
PURPLE acid phosphatases; ARABIDOPSIS thaliana; LEAF aging; FUNCTIONAL genomics; PHOSPHORUS; CHEMICAL composition of plants
- Publication
New Phytologist, 2012, Vol 196, Issue 4, p1024
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.12006