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- Title
NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network.
- Authors
Kano, Fumi; Kondo, Hisao; Yamamoto, Akitsugu; Kaneko, Yayoi; Uchiyama, Keiji; Hosokawa, Nobuko; Nagata, Kazuhiro; Murata, Masayuki
- Abstract
The endoplasmic reticulum (ER) has a characteristic polygonal structure with hallmark three-way junctions. In a previous paper, we reconstituted the disruption of the pre-existing ER network using mitotic cytosol from HeLa cells in streptolysin O (SLO)-permeabilized CHO-HSP cells (stably expressing GFP-HSP47). In addition, we found that interphase cytosol induced reformation of the disrupted ER network into a continuous network structure. Here, we show that the reformation of the ER network is accomplished through two sequential fusion reactions. The first process is mediated by NSF/α and γ-SNAPs, and involves the generation of typical membranous intermediate structures that connect the disrupted ER tubules. A subsequent fusion is mediated by p97/p47/VCIP135, which has been shown to be required for homotypic fusion events in Golgi cisternae regrowth after mitosis. In addition, we also found that both fusion processes involve the t-SNARE, syntaxin 18.
- Subjects
ENDOPLASMIC reticulum; ORGANELLES; CELL cycle; CYTOSOL; STREPTOLYSIN
- Publication
Genes to Cells, 2005, Vol 10, Issue 10, p989
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1111/j.1365-2443.2005.00894.x