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- Title
Effects of physiological and synthetic IAP antagonism on c-IAP-dependent signaling.
- Authors
Kocab, A J; Veloso, A; Paulsen, M T; Ljungman, M; Duckett, C S
- Abstract
Cellular inhibitor of apoptosis proteins 1 and 2 (c-IAP1/2) have central roles in signal transduction mediated by numerous receptors that participate in inflammatory and immune responses. In certain pathways, such as activation of NF-κB, their degradation is a major regulatory event and is physiologically induced by activation of receptors. In addition, a number of synthetic compounds have been developed that also target the c-IAPs and induce their degradation. However, the extent of a synthetic IAP antagonist's ability to mirror the transcriptional program by a physiological signal remains unclear. Here we take a systems approach to compare the transcriptional programs triggered by activation of CD30, a well-characterized receptor previously shown to induce the degradation of the c-IAPs, to SM-164, a synthetic IAP antagonist that specifically triggers c-IAP degradation. Employing a technique that allows the specific analysis of newly transcribed RNA, we have generated comparative transcriptome profiles for CD30 activation and SM-164 treatment. Analysis of these profiles revealed that the genes regulated by each stimulus were not completely shared, indicating novel functions of IAP antagonists and consequences of c-IAP1/2 degradation. The data identified a role for c-IAP1/2 in the regulation of the ribosome and protein synthesis, which was subsequently confirmed by biological assays. These findings expand our knowledge of the roles of c-IAP1/2 in signaling and provide insight into the mechanism of synthetic IAP antagonists, furthering our understanding of their therapeutic potential.
- Subjects
NF-kappa B; APOPTOSIS inhibition; CELLULAR signal transduction; BIODEGRADATION; PHYSIOLOGICAL effects of proteins; IMMUNE response
- Publication
Oncogene, 2015, Vol 34, Issue 43, p5472
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/onc.2015.3