We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks.
- Authors
Horikawa, Izumi; Sedelnikova, Olga A.; Zimonjic, Drazen B.; Popescu, Nicholas C.; Bonner, William M.; Barrett, J. Carl
- Abstract
Humans and animals undergo ageing, and although their primary cells undergo cellular senescence in culture, the relationship between these two processes is unclear. Here we show that ?-H2AX foci (?-foci), which reveal DNA double-strand breaks (DSBs), accumulate in senescing human cell cultures and in ageing mice. They colocalize with DSB repair factors, but not significantly with telomeres. These cryptogenic ?-foci remain after repair of radiation-induced ?-foci, suggesting that they may represent DNA lesions with unrepairable DSBs. Thus, we conclude that accumulation of unrepairable DSBs may have a causal role in mammalian ageing.
- Subjects
DEVELOPMENTAL biology; CELLULAR aging; DNA; CYTOLOGICAL research; TELOMERES; CELL culture
- Publication
Nature Cell Biology, 2004, Vol 6, Issue 2, p168
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1095