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- Title
Freezing of PCR master mixture retains full amplification activity and facilitates PCR standardisation for molecular diagnostics and real-time quantitative PCR.
- Authors
Smetsers, T F C M; Stevens, E H P; van de Locht, L T F; Mensink, E J B M; Smetsers, T F; Stevens, E H; van de Locht, L T; Mensink, E J
- Abstract
Reports that freezing of polymerase chain reaction (PCR) master mixture retains full amplification activity and facilitates PCR standardisation for molecular diagnostics and real-time quantitative PCR. Procedure for the experiment; Possibilities for storing PCR mixtures for enhanced reproducibility; Advantages of the freezing.
- Subjects
POLYMERASE chain reaction; MIXTURES; CRYOBIOLOGY; MOLECULAR diagnosis; CELL lines; COMPARATIVE studies; FREEZING; RESEARCH methodology; MEDICAL cooperation; RESEARCH; RESEARCH evaluation; WEIGHTS &; measures; EVALUATION research
- Publication
Leukemia (08876924), 1998, Vol 12, Issue 8, p1324
- ISSN
0887-6924
- Publication type
letter
- DOI
10.1038/sj.leu.2401082