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- Title
Bt Toxin Modification for Enhanced Efficacy.
- Authors
Deist, Benjamin R.; Rausch, Michael A.; Fernandez-Luna, Maria Teresa; Adang, Michael J.; Bonning, Bryony C.
- Abstract
Insect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for pest suppression. Here we review the different strategies that have been employed to enhance toxicity against specific target species including those that have evolved resistance to Bt, or to modify the host range of Bt crystal (Cry) and cytolytic (Cyt) toxins. These strategies include toxin truncation, modification of protease cleavage sites, domain swapping, site-directed mutagenesis, peptide addition, and phage display screens for mutated toxins with enhanced activity. Toxin optimization provides a useful approach to extend the utility of these proteins for suppression of pests that exhibit low susceptibility to native Bt toxins, and to overcome field resistance.
- Subjects
TOXICOLOGY of Bacillus thuringiensis; PEST control; PLANT protection; MUTAGENESIS; BACTERIOPHAGES
- Publication
Toxins, 2014, Vol 6, Issue 10, p3005
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins6103005