We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Amino Acid Sequence of the Protease Inhibitor BWI-4a From Buckwheat Seeds.
- Authors
Belozersky, Mikhail A.; Dunaevsky, Yakov E.; Musolyamov, Alexander Kh.; Egorov, Tsezi A.
- Abstract
The complete amino acid sequence of protease inhibitor BWI-4a from buckwheat (Fagopyrum esculentum Moench) seeds, consisting of 67 amino acid residues with a single disulfide bond, has been established by Edman degradation in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Its N terminus is blocked by a pyroglutamic acid residue. Mass spectrometric analysis revealed that inhibitor BWI-4a is present in buckwheat seeds in two isoforms with a single amino acid substitution of Ala[sup 40] for Gly[sup 40]. The reactive site of the inhibitor contains an Arg[sup 43]-Asp[sup 44] bond. Analysis of the amino acid sequence suggests that the buckwheat seed protease inhibitor is a member of the potato proteinase inhibitor I family.
- Subjects
AMINO acid sequence; BUCKWHEAT; MASS spectrometry
- Publication
IUBMB Life, 2000, Vol 49, Issue 4, p273
- ISSN
1521-6543
- Publication type
Article
- DOI
10.1080/15216540050033122